Journal: Scientific Reports

Article Title: The Nek2 centrosome-mitotic kinase contributes to the mesenchymal state, cell invasion, and migration of triple-negative breast cancer cells

doi: 10.1038/s41598-021-88512-0

Figure Lengend Snippet: Nek2 induces mRNA level changes in EMT transcription factors. ( a,b ) qPCR analysis of EMT genes in MDA-231 and Hs578t cells. ( c–f ) Invasion of MDA-MB-231 or Hs578t treated with siSlug or siZeb1. ( g–j ) Migration of MDA-MB-231 or Hs578t treated with siSlug or siZeb1.

Article Snippet: Gene expression levels were measured using RT 2 qPCR primers assay (BioRad 1708880) using Qiagen primers for human CDH1/E-cadherin (PPH00135F-200), CDH2/N-cadherin (PPH00636F-200), CTNNB1/β-catenin (PPH00643F-200), SNAI1/Snail (PPH02459B-200), SNAI2/Slug (PPH02475A-200), TWIST1 (PPH02132A-200), FN1/Fibronectin (PPH00143B-200), VIM (PPH00417F-200), TJP1/ZO-1 (PPH09919F-200), ZEB1/Zeb1 (PPH01922A-200), ZEB2/Zeb2 (PPH09021B-200), and GAPDH (PPH00150F-200).

Techniques: Migration

Journal: Lipids in Health and Disease

Article Title: Modulation Peroxisome Proliferators Activated Receptor alpha (PPAR α) and Acyl Coenzyme A: Cholesterol Acyltransferase1 (ACAT1) Gene expression by Fatty Acids in Foam cell

doi: 10.1186/1476-511X-8-38

Figure Lengend Snippet: The effects of fatty acids (100 μM), Wy14643 (50 μmol/L) and Sandoz58-035 (5 μM) on mRNA levels of ACAT1 in THP-1 macrophages derived foam cells . Cells were treated for 48 h with fatty acids and pharmacological compounds. mRNA levels analyzed by cyber green procedures. The values normalized to β-actin. All results represent means ± SD from triplicate determinations, representative of 3 independent experiments compared with control. Significant differences between treatments are indicated by one-way ANOVA followed by Tukey multicomparison test. *P ≤ 0.05.

Article Snippet: SYBER Green PCR Master Mix Reagent and RT 2 qPCR Primeres (ACAT1, PPARα, β-Actin) purchased from (SuperArray Bioscience Corpotation, USA).

Techniques: Derivative Assay

Journal: Lipids in Health and Disease

Article Title: Modulation Peroxisome Proliferators Activated Receptor alpha (PPAR α) and Acyl Coenzyme A: Cholesterol Acyltransferase1 (ACAT1) Gene expression by Fatty Acids in Foam cell

doi: 10.1186/1476-511X-8-38

Figure Lengend Snippet: The effects of fatty acids (100 μM) and pharmacological PPARα and ACAT1 ligands on mRNA levels of PPARα in THP-1 macrophages derived foam cells . Cells were treated for 48 h with fatty acids and pharmacological compounds. mRNA levels analyzed by cyber green procedures. The values normalized to β-actin. All results represent means ± SD from triplicate determinations, representative of 3 independent experiments compared with control. Significant differences between treatments are indicated by one-way ANOVA followed by Tukey multicomparison test. *P ≤ 0.05.

Article Snippet: SYBER Green PCR Master Mix Reagent and RT 2 qPCR Primeres (ACAT1, PPARα, β-Actin) purchased from (SuperArray Bioscience Corpotation, USA).

Techniques: Derivative Assay